Cumulative expression of the two Lyn isoforms creates a threshold for macrophage signaling in the absence of receptor engagement

Clicks: 12
ID: 330751
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #66 of 100 articles by views in journal of leukocyte biology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
It has been unclear whether phenotypic differences between LynA and LynB knockout mice, each expressing only one splice form of the Src-family kinase (SFK) Lyn, are due to isoform-specific functions or dose effects from differences in overall Lyn expression. To assess the functions of LynA and LynB, we generated a genetic series in which LynA and LynB expression levels vary independently, allowing us to test whether upregulation of one Lyn isoform could compensate for loss of the other. We used macrophages from this dose series to test signaling at steady state and in response to pharmacological pan-SFK activation. We found that LynA and LynB are equally capable of phosphorylating the positive-regulatory tyrosine kinase Syk and the negative-regulatory inositol phosphatase SHIP1, to a level determined by the cumulative expression level of LynA + LynB at steady state. Despite the dose-dependence of steady-state and induced phosphorylation of upstream signaling intermediates, Erk and Akt signaling in response to pan-SFK activation is enabled by any amount of Lyn expression, insensitive to further upregulation of either isoform. Thus, expression of either LynA or LynB at steady state leads to balanced potential for positive-regulatory and negative-regulatory signaling, setting a maximal response in the absence of a true microbial encounter.
Reference Key
openalex_W7215116792 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors S. Erandika Senevirathne, Siyu Jin, Carter Sellner, Silvia Toledo Ramos, Tanya S. Freedman
Journal journal of leukocyte biology
Year 2026
DOI
10.1093/jleuko/qiag133
URL
https://doi.org/10.1093/jleuko/qiag133
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.