Biomechanical regulation of Ca2+ dynamics during muscle stem cell activation
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ID: 328297
2026
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Abstract
Muscle satellite cells (MuSCs) are muscle-resident stem cells that are responsible for myofiber regeneration. Although the importance of calcium ions (Ca 2+) in muscle physiology has been well established, the mechanism by which Ca 2+ mobilization governs MuSC function remains poorly understood. In this study, we aimed to systematically characterize Ca2+ dynamics in MuSCs and to define the mechanisms regulating these signals during muscle regeneration. By employing modified protocols for mouse MuSC isolation and Ca2+ measurement, we observed spontaneous Ca2+ fluctuations in MuSCs isolated from regenerating muscle after cardiotoxin-induced myofiber injury. Our detailed analysis using chemical Ca2+ indicators and a genetically encoded Ca2+ indicator revealed that the frequency and amplitude of Ca2+ fluctuations increased significantly during the activated and proliferative stages of MuSCs. This effect was more pronounced in MuSCs isolated from dystrophic and aged mice. Mechanistically, these Ca2+ fluctuations were at least partially mediated by mechanosensitive ion channels, including PIEZO1 and TRPM7, which promote MuSC migration. Collectively, our findings demonstrate that Ca2+ fluctuations through mechanosensitive ion channels act as a key regulator of MuSC activation during muscle regeneration and may provide new insights into the role of Ca2+ influx in muscle biology and the pathogenesis of muscle diseases.
| Reference Key |
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| Authors | Kotaro Hirano, Yudai Ishikawa, Norio Motohashi, Yujiro Kobata, Hiroto Watanabe, Mao Sasaki, Takuya Yokoyama, Yuto Yamada, Yuta Aoyagi, Kanako Takakura, Akira Murakami, Masaki Tsuchiya, Yusuke Ono, Keiko Nonomura, Yoshitsugu Aoki, Yuji Hara |
| Journal | The Journal of Biochemistry |
| Year | 2026 |
| DOI |
10.1093/jb/mvag062
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| URL | |
| Keywords | Keywords not found |
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