Expression analysis and characterization of dmrt2 in Chinese tongue sole (Cynoglossus semilaevis).

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ID: 32783
2019
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Abstract
Dmrt2 is a member of the dmrt gene family with a conserved zinc finger-like DNA-binding motif (DM domain). In the present study, CS-dmrt2 was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of CS-dmrt2 is 2834 bp in length, with a 251 bp 5'-untranslated region (UTR), a 1086 bp 3'-UTR and a 1503 bp open reading frame (ORF) that encodes a 501-amino-acid peptide. qPCR revealed that CS-dmrt2 was mainly expressed in C. semilaevis testes. In situ hybridization (ISH) showed CS-dmrt2 expression throughout early gonadal development (36 days after hatching (dah) and 86 dah), but the expression was higher in male gonads than in female gonads. CS-dmrt2 mRNA was highly expressed in male germ cells. Comparison of methylation levels between females and males demonstrated hypo-methylated levels of the CS-dmrt2 promoter in the male gonads, which is consistent with the high mRNA expression. These results suggest that the CS-dmrt2 gene may play a functional role in gonadal differentiation/development and germ cell maturation in the testis.
Reference Key
zhu2019expressiontheriogenology Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhu, Ying;Cui, Zhongkai;Yang, Yingming;Xu, Wenteng;Shao, Changwei;Fu, Xiaoqin;Li, Yangzhen;Chen, Songlin;
Journal Theriogenology
Year 2019
DOI
S0093-691X(19)30258-4
URL
Keywords Keywords not found

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