GRIDGENE: Guided Region Identification based on Density of GENEs – a transcript density-based approach to characterize tissues by spatial transcriptomics

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ID: 326434
2026
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Abstract
Abstract Spatial omics brought unprecedented power to study biological processes within tissues while preserving spatial context and morphology. Most spatial proteomics and transcriptomics analysis methods are cell-centric, relying on cell segmentation to identify and characterize individual cells before downstream tasks. However, certain biological questions may be better addressed using cell-free approaches, which also eliminate unnecessary computations when cell segmentation is not essential. To address this need, we developed GRIDGENE (Guided Region Identification based on Density of GENEs), an approach for defining regions of interest based on transcript density. GRIDGENE enables the identification of biologically relevant tissue compartments, including interfaces between regions, phenotype-enriched areas, and zones defined by specific gene signatures, supporting analyses such as pathway enrichment. We demonstrated the utility of GRIDGENE by applying it to spatial transcriptomics data from CosMx and Xenium platforms in colorectal cancer samples. By bypassing cell segmentation, our approach enables flexible analysis of spatial omics data, supporting the study of biological processes across diverse tissue structures and microenvironments. Nevertheless, GRIDGENE can be easily integrated with cell segmentation strategies for complementary analyses. GRIDGENE thus broadens the analytical toolkit for spatial omics, enabling both cell-free and cell-based insights.
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openalex_W7204210584 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors A M Sequeira, M E Ijsselsteijn, M Rocha, J Roelands, Noel F C C de Miranda
Journal Bioinformatics advances
Year 2026
DOI
10.1093/bioadv/vbag249
URL
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