Evaluation of a Direct-from-Blood Molecular Diagnostic Platform for Pathogen Detection in Critically Ill Patients: A Prospective Observational Cohort Study

Clicks: 4
ID: 324974
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #81 of 207 articles by views in Open forum infectious diseases

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 207 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Rationale Defining the microbial etiology of sepsis is difficult but has important implications for antibiotic stewardship and prognosis. We explore the diagnostic yield of a novel, direct–from-blood multiplex PCR in ICU patients with suspected sepsis. Methods From March 2022 to March 2024, patients were included if the clinical team ordered a blood culture. In study participants, an additional 6 mL of whole blood was collected by the phlebotomist. Samples were analyzed by the RaPID/BSI platform, a PCR-based assay that detects 19 bacterial and fungal species direct from whole blood. Results 165 samples were included in the final cohort. Of these, 27 patients had a positive RaPID/BSI result and 8 patients had positive blood cultures with one pathogen not included in the RaPID/BSI panel. Of the 27 patients with positive RaPID/BSI results, 5 patients also had positive blood cultures. Pathogen identification from RaPID/BSI and blood cultures were concordant for 4 out of 5 of these pathogens. 25 of the 27 RaPID/BSI positive patients had clinically defined infections. 18 patients of the 27 RaPID/BSI positive patients had additional positive microbial culture data that corroborated pathogen identification. Of the 27 patients with bloodstream infections detected by RaPID/BSI and/or blood cultures, 20 had received antibiotics in the 48 hours prior to their blood draw. The RaPID/BSI assay identified the pathogen in all 20 of these patients, whereas only 4 out of these 20 patients had positive blood cultures. Conclusion The diagnostic yield of a direct-from-blood molecular diagnostic assay for the detection of bloodstream infection was 15.2% compared to 4.3% from blood cultures, providing higher detection rate especially in the setting of recent antibiotic administration.
Reference Key
openalex_W7202348418 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Yupeng Liu, Helen Donnelly, Alvaro Donayre, Erin Korth, Bridget Giblin, Francisco Martínez, Katie Clepp, Richard G. Wunderink, Chiagozie I. Pickens
Journal Open forum infectious diseases
Year 2026
DOI
10.1093/ofid/ofag486
URL
https://doi.org/10.1093/ofid/ofag486
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.