The OsMYB103-OsGLOX5 module regulates tapetum degradation and male fertility in rice

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ID: 324849
2026
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Abstract
Abstract Anther tapetum degradation is essential for normal pollen formation in rice, yet the underlying regulatory network remains poorly understood. We isolated a novel male sterility rice mutant, osglox5, and confirmed that the target gene LOC_Os11g06870 encodes a glyoxal/galactose oxidase protein with both glyoxal oxidase (GLOX) and galactose oxidase (GAO) activities and is required for hydrogen peroxide (H2O2) production in anthers. Insufficient H2O2 content in osglox5 anthers causes delayed tapetum degradation and defective pollen wall formation, resulting in pollen abortion. OsGLOX5 is highly expressed during stage 9 of anther development, specifically in microspores and tapetum, and localizes to the endoplasmic reticulum. The tapetum-specific transcription factor OsMYB103 directly binds the OsGLOX5 promoter and activates its expression. This study provides the first evidence that a protein with both glyoxal oxidase and galactose oxidase activities plays an essential role in rice tapetum degradation, and identifies a novel MYBs-GLOXs regulatory axis, advancing our understanding of the tapetum degradation network.
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openalex_W7202367069 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Weichi Liu, Jie Yang, Yuan Chen, Yongqiao Long, Shuqing Lai, Kaiwen Chen, Yanlin Chen, Juan Rao, Junqiu Liang, Hongxia Yang, Yao Deng, Yingchun Wan, Lin Zhang, Guanghua He, Yudong Jiang, Nan Wang, Wenqiang Shen, Ping Feng
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag608
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