Mouse NK cell functional maturation is dependent on MHC I

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ID: 324680
2026
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Abstract
Abstract NK cells effectively eliminate infected cells or tumour cells. The acquisition of cytotoxic functions occurs throughout NK cell functional maturation, a process that is commonly defined by the sequential expression of CD27 and CD11b. In addition, NK cell cytotoxicity is finely tuned by the process of education, which is dependent on MHC I. Expression of effector molecules is not impaired in NK cells from MHC I-deficient mice, but their cytotoxic activity towards target cells is reduced. While the role of MHC I on NK cell education has been extensively studied, its impact on NK cell functional maturation has not been defined. Using two distinct MHC I-deficient mouse models, we confirm that MHC I is not required for expression of effector molecules by NK cells. However, based on CD27 and CD11b expression, we find that fewer NK cells reach the final stage of NK cell functional maturation. Moreover, in β2m-deficient mice, early-stage NK cells express higher levels of granzyme B. Together, these findings demonstrate that NK cell functional maturation is dependent on MHC I and that the expression of effector proteins can be uncoupled from the NK cell functional maturation stages.
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openalex_W7202201166 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Capucine Bourel, Félix Lombard‐Vadnais, Sarah Pasquin, Sylvie Lesage
Journal journal of leukocyte biology
Year 2026
DOI
10.1093/jleuko/qiag114
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