The PtARF6/PtARF8-PtGH3.1 transcriptional module regulates auxin homeostasis and dwarfism in citrus

Clicks: 3
ID: 323607
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #157 of 498 articles by views in Plant physiology and biochemistry : PPB

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 498 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Dwarfing rootstocks are crucial for high-density and labor-efficient citrus cultivation, yet the molecular mechanisms underlying plant height regulation remain poorly understood. Using an integrated multi-omics analysis of dwarf and normal-height red tangerine (Citrus reticulata) × trifoliate orange (Poncirus trifoliata) hybrid seedlings, we identified the Gretchen Hagen 3.1 gene PtGH3.1, an auxin-conjugating enzyme, as an important regulator of dwarfism. Compared with normal-height hybrid seedlings, PtGH3.1 was significantly upregulated in the shoot tips of dwarf hybrid seedlings, correlating with reduced free auxin (indole-3-acetic acid, IAA) levels. Functional studies in a model citrus system (Citrus macrocarpa) demonstrated that heterologous overexpression of PtGH3.1 decreased free IAA and inhibited plant growth, whereas CRISPR/Cas9-mediated knockout of its homolog CmGH3.1 with editing ratio below 40% promoted growth. Mechanistically, the transcription factors auxin response factor 6 (PtARF6) and auxin response factor 8 (PtARF8) physically interact, forming a heterodimer that activates PtGH3.1 transcription. Dwarf hybrid seedlings exhibited elevated PtARF6 but reduced PtARF8 transcript levels. Heterologous overexpression of either PtARF6 or PtARF8 in Citrus macrocarpa induced dwarfism by activating CmGH3.1 expression. This study reveals a PtARF6/8-PtGH3.1 transcriptional module that regulates auxin homeostasis through IAA conjugation, providing genetic insights into GH3.1-mediated dwarfism and identifying precise targets (PtGH3.1, PtARF6, and PtARF8) for breeding dwarfing rootstocks in citrus.
Reference Key
openalex_W7172388600 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Shimin Chen, Zhengyan Fan, Jiaxuan Peng, Haiguang Zheng, Qiang Xu, Shunyuan Xiao, Zhiyong Pan
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag561
URL
https://doi.org/10.1093/plphys/kiag561
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.