Dynamic Expression of α-1,2-Glucan Synthase in Nostoc sp. PCC 7120 and Biochemical Characterization of an α-1,2-Glucan phosphorylase

Clicks: 31
ID: 323598
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #26 of 75 articles by views in glycobiology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
α-1,2-glucans are polysaccharides characterized by their unique α-1,2-glycosidic bonds and right-handed helical structure, which suggest novel biological functions. The alr1000 protein in Nostoc sp. PCC 7120 is the key enzyme for synthesizing α-1,2-glucans. In this study, we prepared rabbit antiserum for alr1000 and investigated its expressions when Nostoc sp. PCC 7120 is subjected to high salinity and high temperature stress. The results showed that under high salinity (up to 200 mM) and high temperature (40°C) conditions, alr1000 expression reached its highest level at 4 hours. This suggests that the elevated expression of alr1000 initiates massive production of α-1,2-glucans within Nostoc sp. PCC 7120, which helps the organism survive these stressful conditions. In addition, we identified and characterized the degrading enzymes of α-1,2-glucans. All4989, a GH65 family α-1,2-glucan phosphorylase from Nostoc sp. PCC 7120, was found to degrade α-1,2-glucans through a mechanism where a phosphate serves as the nucleophile to cleave the α-1,2-glycosidic bonds of α-1,2-glucans, while Glu485 functions as a general acid. Furthermore, the enzyme also catalyzes an efficient reverse reaction, synthesizing α-1,2-glucans of different molecular weights using breakdown products as substrates. Mechanistically, this process involves a key functional shift: Glu485 acts as a general acid during the forward degradation and switches to acting as a general base during the reverse synthesis.
Reference Key
openalex_W7172423383 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Qinghui An, Hina Farooq, Anqi Wang, Yuyang Lou, Mengli Liu, Jiyong Su
Are you one of these authors? Claim your profile
Journal glycobiology
Year 2026
DOI
10.1093/glycob/cwag061
URL
https://doi.org/10.1093/glycob/cwag061
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.