The Dof20 transcription factor activates SRPP7 expression to promote natural rubber biosynthesis in Taraxacum kok-saghyz

Clicks: 1
ID: 321493
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #486 of 501 articles by views in Plant physiology and biochemistry : PPB

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 501 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract DNA binding with one finger (Dof) transcription factors play vital roles in plant growth, development, and secondary metabolism, yet their involvement in natural rubber (NR) biosynthesis remains unknown. Natural rubber is an indispensable industrial biopolymer widely used in transportation, medicine, and advanced manufacturing. Here, we identified a Dof transcription factor, TkDof20, as a latex-specific, nucleus-localized activator in Taraxacum kok-saghyz. Overexpression of TkDof20 significantly increased latex yield, laticifer area, and NR content, accompanied by a substantial reduction in inulin accumulation. Correspondingly, overexpression of TkSRPP7, encoding a small rubber particle protein (SRPP), also enhanced NR accumulation. The tksrpp5 tksrpp6 tksrpp7 tksrpp10 tksrpp11 quintuple mutant (tksrpp△5) displayed a pronounced reduction in both rubber yield and laticifer number. Yeast one-hybrid and dual-luciferase assays demonstrated that TkDof20 directly bound to the AAAG cis-element within the TkSRPP7 promoter, thereby activating its transcription. These findings establish a Dof-SRPP regulatory module that promotes rubber accumulation by reinforcing rubber particle formation. Comparative analyses further revealed that SRPP homologs from Hevea brasiliensis and Eucommia ulmoides shared conserved sequence features, subcellular localization, and biological functions. Ectopic expression of HbSRPP4 and EuSRPP4 in T. kok-saghyz significantly enhanced NR yield, and their corresponding transcription factors, HbDof18 and EuDof29, similarly activated SRPP promoters. Collectively, this study identifies TkDof20 as an upstream regulator of SRPP-mediated NR biosynthesis and reveals a Dof-SRPP transcriptional module involved in latex formation. These discoveries provide insights into the molecular regulation of natural rubber biosynthesis and offer promising genetic targets for the metabolic engineering of high-yield rubber crops.
Reference Key
openalex_W7169542359 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fengyan Fang, Hongzhi Ge, M L Wang, Xiaoyou Wu, Jintao Li, Shouling Li, Wei Li, Shugang Hui, Xuchu Wang
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag520
URL
https://doi.org/10.1093/plphys/kiag520
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.