Constitutive mTORC1 Activation in Skeletal Muscle Increases Inflammation but is not Sufficient to Impair Glucose Tolerance

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ID: 321367
2026
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Abstract
Aberrant mechanistic target of rapamycin complex 1 (mTORC1) signaling in skeletal muscle has been implicated in aging and insulin resistance, however, it is not known whether chronic mTORC1 activation directly causes glucose intolerance. We tested the hypothesis that constitutive mTORC1 activation in mouse skeletal muscle impairs glucose homeostasis. Six-month-old female and male mice with tamoxifen-inducible, muscle-specific knockout of Depdc5, a key component of the GATOR1 complex and negative regulator of mTORC1, were fed normal chow or western diet (WD; 45% fat, 17% sucrose) for 12 weeks. Depdc5 knockout (KO) increased mTORC1 signaling and altered autophagy markers. WD increased body and fat mass and impaired glucose tolerance independent of genotype. KO had minimal effects on fasting glucose, insulin, HOMA-IR, HbA1c, or oral glucose tolerance, although female KO mice showed a modest increase in WD-induced weight gain and fasting glucose. Mitochondrial respiration and content were unchanged by KO or WD. KO increased mitochondrial Hâ‚‚Oâ‚‚ production capacity but did not drive clear signs of oxidative stress. Transcriptomic analysis revealed robust KO-driven upregulation of genes related to cell division and immune pathways. Consistent with this, KO increased TNF-α and IL-6 protein expression and shifted macrophage polarization toward an M2-like phenotype without altering total macrophage content. Collectively, these findings indicate that chronic activation of mTORC1 in skeletal muscle promotes inflammatory remodeling but is insufficient to impair systemic glucose homeostasis, even under dietary stress.
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Authors Erik D. Marchant, Hanna Kalenta, Sean P. Kilroe, Jacquelyn May, Wenjun Z. Martini, Korri Weldon, Zhao Lai, Blake B. Rasmussen
Journal Food & function
Year 2026
DOI
10.1152/function.039.2026
URL
https://doi.org/10.1152/function.039.2026
Keywords Keywords not found

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