Natural cross-kingdom transmission of a novel ssDNA mycovirus confers broad-spectrum resistance to plant diseases

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ID: 319346
2026
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Abstract
Cross-kingdom virus transmission between plants and fungi remains rare in nature. Here, a novel circular ssDNA virus, Diaporthe pseudophoenicicola DNA virus 1 (DpDV1), was identified from the phytopathogenic fungus Diaporthe pseudophoenicicola. DpDV1 attenuates the pathogenicity of both its original and experimental host fungi, demonstrating its potential as a biocontrol agent against fungal phytopathogens. Remarkably, DpDV1 displays bidirectional plant-fungal transmission capability without inducing observable phenotypic changes in plant hosts, overcoming the limitations imposed by vegetative incompatibility in conventional hypovirulence-based biocontrol approaches. Immunocytochemical localization assays revealed DpDV1 is localized in the chloroplasts of plant cells, demonstrating its cross-kingdom transmission. Furthermore, we demonstrated DpDV1 can cross-protect plants against plant viruses, establishing foundations for cross-protection-based management of plant virus diseases. Hence, we report a novel ssDNA mycovirus and provide evidence of mycovirus cross-kingdom transmission between plant and fungi, providing innovative strategies for mycovirus biocontrol of plant fungal and viral diseases.
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openalex_W7166804437 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Siyu Zhou, Yuanhao Sun, Peng Li, Xuetuan Lin, Xiaofei Liang, Jie Zhou, Jiǎtāo Xiè, Li Zheng
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag464
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