ClearDepthIAS enables automated high-throughput quantification of roots in soil-grown taproot crops

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ID: 318910
2026
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Ranked #482 of 502 articles by views in Plant physiology and biochemistry : PPB

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Abstract
Abstract Understanding root system architecture (RSA) is critical for improving crop productivity and resilience, yet phenotyping root traits such as root growth angle and rooting depth remains technically challenging, especially at high throughput. Here, we present ClearDepthIAS, a high-throughput imaging and analysis platform that enables non-destructive, automated quantification of root architecture traits in taproot system crops. By capturing and stitching 360° images of roots growing along the transparent walls of pots and applying deep learning–based segmentation (ClearDepth-WRT), we measured wall root shallowness (WRS)—a proxy for root growth angle—with high precision. We demonstrated for the tap root systems of soybean and canola that the system accurately detects root tips, quantifies their vertical distribution, and extracts biologically meaningful traits such as root area, distribution indices, and growth angles. Validation experiments in canola and soybean demonstrated that WRS can correlate with root crown architecture in mature plants, both in greenhouse and field settings. Furthermore, WRS and root distribution indices derived from ClearDepthIAS are predictors of early root architecture and can be correlated with root biomass distribution across soil depths under field conditions; however, environmental interactions may influence these relationships and weaken or even negate such correlations, as observed when comparing field to field variation in root system architecture. Our system enables efficient phenotyping of genetically diverse populations, with medium to high trait heritability, supporting its utility for genome-wide association studies and breeding. ClearDepthIAS accelerates the development of root ideotypes for improved resource acquisition and carbon sequestration, offering a scalable tool for supporting climate-resilient agriculture.
Reference Key
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Authors Ashish B. Rajurkar, Lin Wang, Lucas Funaro, Samantha Bellier-Igasaki, Shane Hunt, L Zhang, Avery Talgo, Petra Banuet, Justin Allen, Erin Daniels, Michael Stamm, Wolfgang Busch
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag447
URL
https://doi.org/10.1093/plphys/kiag447
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