Multilayered regulation of chloroplast development by single B-BOX CONSTANS-LIKE and GOLDEN2-LIKE proteins in Arabidopsis
Clicks: 2
ID: 318024
2026
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Emerging Content
0.3
/100
2 views
1 readers
AI Quality Assessment
Not analyzed
Readership in this journal
EmergingRanked #391 of 502 articles by views in Plant physiology and biochemistry : PPB
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 502 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Abstract Chloroplast development is primarily regulated by transcription factors, including those belonging to the GOLDEN2-LIKE (GLK) family, that alter the expression of genes encoding chloroplast proteins. However, the mechanisms by which multiple transcription factors coordinate to optimize chloroplast development remain unclear. In this study, we identified the single B-BOX (BBX) CONSTANS-LIKE (COL) proteins COL6 (also known as BBX14), COL7 (BBX16), COL8 (BBX17), and COL16 (BBX15) as novel regulators of chloroplast development in Arabidopsis thaliana. Notably, the col6 col16 (col-d) double mutant exhibited a chlorophyll-excess phenotype, which was further enhanced in the quadruple col6 col7 col8 col16 (col-q) mutant. Introduction of col-d and col-q mutations in the glk1 glk2 (glk-d) mutant partially restored the pale-green phenotype of the original glk-d mutant. COL proteins negatively and redundantly regulated a class of genes encoding chloroplast proteins, acting directly on their promoters. Moreover, COL6 and COL7 expression exhibited a diurnal cycle, with their expression patterns contrasting with those of GLK1 and GLK2, which are positive regulators of chloroplast development. Furthermore, GLK expression was markedly higher in the col-q mutants than in wild-type plants. Co-immunoprecipitation assays revealed that COL proteins interacted with the GARP DNA-binding domain in the N-terminus of GLK1, likely affecting its function. Moreover, COL proteins associate with each other to form homomeric and heteromeric complexes, suggesting functional redundancies at the protein level. In conclusion, these results suggest that the multilayered regulatory mechanisms of single BBX COL and GLK proteins optimize chloroplast development in Arabidopsis thaliana in fluctuating environments.
| Reference Key |
openalex_W7165406340
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | 角田 浩太郎, Mari Abumi, Hiroko Kinoshita, Mami Higashi, Hibiki Shiiba, Shiho Shimizu, Shogo Kuramoto, Fumika Nishida, Karin Kawajiri, Kagari Sakugawa, Mayuko Sato, Kiminori Toyooka, Yasuko Ito‐Inaba, Takehito Inaba |
| Journal | Plant physiology and biochemistry : PPB |
| Year | 2026 |
| DOI |
10.1093/plphys/kiag406
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.