Lignin structural changes and high p -coumaroylation in incipient lignification in moso bamboo

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ID: 318007
2026
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Abstract
Lignification is a crucial process for strengthening plant tissues, facilitating water transport, and providing defense against pathogens. In the Poaceae family, p-hydroxycinnamic acids are commonly incorporated into lignin, with acylation by p-coumarate (pCA) occurring during lignification. In this study, we performed DFRC and 2D HSQC-NMR analyses to investigate changes in lignin substructures and the degree of lignin pCA-acylation throughout bamboo stem development. Furthermore, immunohistochemical analysis was conducted to elucidate the spatial distribution of lignin substructures within different cell types. Our results revealed that, in young tissues, β-O-4-linked lignin units are predominantly derived from monolignol-pCA conjugates, specifically coniferyl- and sinapyl-pCA. Both lignin structure and the pattern of pCA acylation varied depending on the stage of cell wall formation and the cell type, particularly between vascular fiber cells and parenchyma cells. Based on our results, moso bamboo culms exhibit a distinctive feature during incipient lignification, in which monolignols are predominantly acylated with pCA. This feature has not been reported in other grasses, suggesting that extensive p-coumaroylation of monolignols plays an important role in the rapid elongation of bamboo culms.
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Authors Noriaki Munekata, Steven D. Karlen, Arata Yoshinaga, Taku Tsuyama, Rebecca A. Smith, Fabienne Guillon, Tatsuya Awano, John Ralph, Junji Sugiyama
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag407
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