Calcium channel CNGC20 and calmodulin 7 regulate melatonin-induced calcium signaling and aphid resistance in watermelon
Clicks: 1
ID: 317335
2026
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
0.0
/100
1 views
0 readers
AI Quality Assessment
Not analyzed
Readership in this journal
Ranked #408 of 501 articles by views in Plant physiology and biochemistry : PPB
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 501 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Melatonin plays a crucial role in regulating plant defense against various environmental stresses. However, its role and the underlying mechanism in enhancing plant resistance to aphid infestation remains elusive. In this research, we discovered that aphid infestation notably upregulated the expression of the melatonin biosynthetic gene caffeic acid O-methyltransferase 1 (ClCOMT1) and increased melatonin levels in watermelon (Citrullus lanatus). The overexpression of ClCOMT1 enhanced defensive responses and resistance to aphids in watermelon. This was accompanied by the accumulation of cytosolic free calcium ([Ca2+]cyt), upregulation of the Ca2+-permeable channel gene cyclic nucleotide-gated ion channel 20 (ClCNGC20), and downregulation of calmodulin 7 (ClCaM7). ClCaM7 interacts with ClCNGC20 and suppresses its channel activity. In contrast, the knockout of ClCOMT1 showed opposite effects compared with its overexpression. Pretreatment with LaCl3 (a Ca2+ influx channel inhibitor) or EGTA (a Ca2+ chelator) compromised ClCOMT1 overexpression-induced aphid resistance; whereas, CaCl2 or the Ca2+ ionophore A23187 effectively restored aphid resistance in clcomt1 mutant. Moreover, silencing ClCNGC20 impaired ClCOMT1 overexpression-induced [Ca2+]cyt accumulation and aphid resistance; conversely, silencing ClCaM7 restored [Ca2+]cyt accumulation and aphid resistance in the clcomt1 mutant. Overexpression of watermelon ClCNGC20 elevated [Ca2+]cyt levels and improved aphid resistance in transgenic Arabidopsis plants, whereas overexpression of watermelon ClCaM7 had the opposite effect. Collectively, these findings imply the crucial role of ClCNGC20 and ClCaM7 in mediating melatonin-induced [Ca2+]cyt elevation, subsequently activating defensive responses in plants. This study offers insights into the molecular mechanisms underlying melatonin-mediated plant aphid resistance, with potential applications in the breeding or engineering of aphid-resistant cucurbit varieties.
| Reference Key |
openalex_W7164758327
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Juezhuo Li, Yuehua Chen, Yi Zhou, Hailiang Yu, Yanliang Guo, Zhongyuan Wang, Wanbang Yang, Chunhua Wei, Jianxiang Ma, Yong Zhang, Jie Yang, Yuan Li, Xian Zhang, Xueyan Zhang, Hao Li |
| Journal | Plant physiology and biochemistry : PPB |
| Year | 2026 |
| DOI |
10.1093/plphys/kiag363
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.