The PbWRKY7-PbMADS2 transcriptional module coordinates volatile ester biosynthesis in pear fruit

Clicks: 1
ID: 316865
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #437 of 502 articles by views in Plant physiology and biochemistry : PPB

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 502 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract Fruit aroma significantly influences fruit quality and shapes consumer preferences. Despite significant advancements in deciphering the metabolic pathways related to volatile compound biosynthesis, the regulatory mechanisms governing these pathways remain relatively underexplored. ‘Fragrant pear’ (Pyrus sinkiangensis Yu) and some pear varieties with Pyrus sinkiangensis lineage possess intense aromas, providing materials for studying the metabolic regulatory pathways of aroma compound biosynthesis. In this study, we used the ‘Chongyanghong’ pear (Pyrus sinkiangensis × Pyrus bretschneideri) as the experimental material. Gas chromatography-mass spectrometry (GC-MS) and RNA sequencing (RNA-Seq) were used to systematically analyze the relationship between metabolites and gene regulation. PbAAT1, an alcohol acyltransferase, was identified as the key enzyme responsible for ester biosynthesis in pears. Multiple experiments collectively confirmed that PbAAT1 was involved in ester synthesis. PbWRKY7 was identified as an important upstream transcriptional activator of PbAAT1 using dual-luciferase, yeast one-hybrid (Y1H), and electrophoretic mobility shift assays. This transcriptional activation effect was further enhanced by the complex formed between PbWRKY7 and the MADS-box protein PbMADS2, as revealed using yeast two-hybrid (Y2H), luciferase complementation (LCA), GST pull-down, dual-luciferase, and transient transformation assays in pears. This study revealed a molecular network in which the PbWRKY7-PbMADS2 interaction synergistically regulates PbAAT1 expression, providing insights into ester biosynthesis and pinpointing regulatory targets for enhancing fruit aroma quality.
Reference Key
openalex_W7164123637 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Fengli Zhou, Hongjuan Zhang, Jingjing Miao, Congcong Wang, X Wang, Haowei Cao, Rui Zhai, Chengquan Yang, Zhigang Wang, Lingfei Xu
Journal Plant physiology and biochemistry : PPB
Year 2026
DOI
10.1093/plphys/kiag346
URL
https://doi.org/10.1093/plphys/kiag346
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.