Uncovering syntrophic potential from genome-resolved metagenomics of suspended and granular AD sludges

Clicks: 3
ID: 314599
2026
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #70 of 78 articles by views in fems microbiology ecology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Syntrophic microbial interactions are fundamental to the degradation of organic matter (e.g. fatty acids), playing a central role in natural anoxic ecosystems and engineered systems such as anaerobic digestion (AD). Despite their ecological and biotechnological importance, only a limited number of (obligate) syntrophic fatty-acid oxidizers have been successfully isolated. In this study, microbial communities from suspended and granular sludge samples were characterized using 16S rRNA gene amplicon sequencing and shotgun metagenomics. Network analysis of the 16S rRNA gene amplicon data revealed strong positive associations between methanogens and known syntrophic fatty-acid oxidizers, particularly in granular sludge samples. 743 High-Completion Metagenome Assembled Genomes (HC-MAGs) were recovered. This comprehensive HC-MAGs dataset provides a valuable resource for identifying novel microorganisms with genomic potential for syntrophic oxidation of butyrate, propionate and acetate. This analysis identified multiple interesting novel targets, including Syntrophomonadia families DTU052 and CALXsZ01 as potential butyrate oxidizers; Syntrophia families UBA6807, PHBD01, FEN-1087, and FEN-1099 as potential propionate oxidizers; and, Thermacetogeniaceae genus DTU068 together with Chloroflexota family 4572-78 as potential acetate oxidizers. These findings highlight granular sludges as a reservoir for previously uncharacterized syntrophic microorganisms. The recovered HC-MAG dataset also provides a framework to further elucidating fatty-acid oxidizing bacterial lineages within complex anaerobic communities.
Reference Key
openalex_W7162026761 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Maaike S. Besteman, Emilie Alaux, Anna Doloman, Guillaume Tahon, Thijs J G Ettema, Diana Sousa
Journal fems microbiology ecology
Year 2026
DOI
10.1093/femsec/fiag052
URL
https://doi.org/10.1093/femsec/fiag052
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.