Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering

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ID: 305907
1990
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Abstract
In N-glycosylated glycoproteins, carbohydrate is attached to Asn in the sequence Asn-X-Ser/Thr, where X denotes any amino acid. However, the presence of this consensus peptide does not always lead to glycosylation. We have compiled an extensive collection of glycosylated and non-glycosylated Asn-X-Thr/Ser sites and present a statistical study based on this data set. Our results indicate that non-glycosylated sites tend to be found more frequently towards the C termini of glycoproteins, and that proline residues in positions X and Y in the consensus Asn-X-Thr/Ser-Y strongly reduce the likelihood of N-linked glycosylation. Beyond this, there are no obvious local sequence features that seem to correlate with the absence or presence of N-linked glycosylation. These findings are discussed in terms of the prediction and engineering of glycosylation sites in secretory proteins.
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openalex_W2049122170 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ylva Gavel, Gunnar von Heijne
Journal Protein Engineering Design and Selection
Year 1990
DOI
10.1093/protein/3.5.433
URL
https://academic.oup.com/peds/article-pdf/3/5/433/4260669/3-5-433.pdf
Keywords Keywords not found

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