Creation of Low-Copy Integrated Transgenic Lines in Caenorhabditis elegans

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ID: 302967
2001
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Abstract
Abstract In Caenorhabditis elegans, transgenic lines are typically created by injecting DNA into the hermaphrodite germline to form multicopy extrachromosomal DNA arrays. This technique is a reliable means of expressing transgenes in C. elegans, but its use has limitations. Because extrachromosomal arrays are semistable, only a fraction of the animals in a transgenic extrachromosomal array line are transformed. In addition, because extrachromosomal arrays can contain hundreds of copies of the transforming DNA, transgenes may be overexpressed, misexpressed, or silenced. We have developed an alternative method for C. elegans transformation, using microparticle bombardment, that produces single- and low-copy chromosomal insertions. Using this method, we find that it is possible to create integrated transgenic lines that reproducibly express GFP reporter constructs without the variations in expression level and pattern frequently exhibited by extrachromosomal array lines. In addition, we find that low-copy integrated lines can also be used to express transgenes in the C. elegans germline, where conventional extrachromosomal arrays typically fail to express due to germline silencing.
Reference Key
openalex_W1911220294 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Vida Praitis, Elizabeth Casey, David C. Collar, Judith Austin
Journal current genetics
Year 2001
DOI
10.1093/genetics/157.3.1217
URL
https://doi.org/10.1093/genetics/157.3.1217
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