Solubilization of Plant Membrane Proteins for Analysis by Two-Dimensional Gel Electrophoresis

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ID: 300910
1986
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Abstract
A plasma membrane-enriched fraction prepared from barley roots was analyzed by two-dimensional gel electrophoresis. Four methods of sample solubilization were assessed on silver stained gels. When membranes were solubilized with 2% sodium dodecyl sulfate followed by addition of Nonidet P-40, gels had high background staining and few proteins because of incomplete solubilization. Gels of membranes solubilized in urea and Nonidet P-40 had a greater number of proteins but proteins with molecular weights greater than 85,000 were absent and proteins with low molecular weights were diffuse. High molecular weight proteins were present in gels of membranes solubilized in 4% sodium dodecyl sulfate followed by acetone precipitation but background staining and streaking remained a problem. Gels of the best quality were obtained when membrane proteins were extracted with phenol and precipitated with ammonium acetate in methanol; background staining and streaking were diminished and proteins were clearly resolved. This method makes possible the resolution required for meaningful qualitative and quantitative comparisons of protein patterns on two-dimensional gels of plant membrane proteins.
Reference Key
openalex_W2061765768 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors William J. Hurkman, Charlene K. Tanaka
Journal Plant physiology and biochemistry : PPB
Year 1986
DOI
10.1104/pp.81.3.802
URL
https://academic.oup.com/plphys/article-pdf/81/3/802/35623018/plphys_v81_3_802.pdf
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