Bacterial magnetosomes-based nanocarriers for co-delivery of cancer therapeutics in vitro

Clicks: 399
ID: 29966
2018
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #15 of 189 articles by views in international journal of nanomedicine

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 189 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Rui-Min Long,1,2,* Qing-Lei Dai,1,* Xia Zhou,1,* Duan-Hua Cai,1 Ya-Zhen Hong,1–3 Shi-Bin Wang,1–4 Yuan-Gang Liu1–3 1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China; 2Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China; 3Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China; 4Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China *These authors contributed equally to this work Abstract: In recent times, co-delivery of therapeutics has emerged as a promising strategy for treating dreadful diseases such as cancer.Materials and methods: In this study, we developed a novel nanocarrier based on bacterial magnetosomes (BMs) that co-loaded with siRNA and doxorubicin (DOX) using polyethyleneimine (PEI) as a cross-linker (BMs/DP/siRNA). The delivery efficiency of siRNA as well as the pH-responsive release of DOX, and synergistic efficacy of these therapeutics in vitro were systematically investigated.Results: The structure of DOX–PEI (DP) conjugates that synthesized via hydrazone bond formation was confirmed by 1H nuclear magnetic resonance (NMR). The in vitro release experiments showed that the DP conjugate (DOX-loading efficiency – 5.77%±0.08%) exhibited the long-term release behavior. Furthermore, the optimal BMs/DP/siRNA particle size of 107.2 nm and the zeta potential value of 31.1±1.0 mV facilitated enhanced cellular internalization efficiency. Moreover, the agarose gel electrophoresis showed that the co-delivery system could protect siRNA from degradation in serum and RNase A. In addition, the cytotoxicity assay showed that BMs/DP/siRNA could achieve an excellent synergistic effect compared to that of siRNA delivery alone. The acridine orange (AO)/ethidium bromide (EB) double staining assay also showed that BMs/DP/siRNA complex could induce cells in a stage of late apoptosis and nanocomplex located in the proximity of the nucleus.Conclusion: The combination of gene and chemotherapeutic drug using BMs is highly efficient, and the BMs/DP/siRNA would be a promising therapeutic strategy for the future therapeutics. Keywords: bacterial magnetosomes, co-delivery, gene therapy, pH-responsive release 
Reference Key
r2018bacterialinternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors R, Long;Q, Dai;X, Zhou;D, Cai;Y, Hong;SB, Wang;Y, Liu;
Journal international journal of nanomedicine
Year 2018
DOI
DOI not found
URL
https://www.dovepress.com/bacterial-magnetosomes-based-nanocarriers-for-co-delivery-of-cancer-th-peer-reviewed-article-IJN
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.