Activation Tagging in Arabidopsis

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ID: 299478
2000
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Abstract
Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants. New activation-tagging vectors that confer resistance to the antibiotic kanamycin or the herbicide glufosinate have been used to generate several tens of thousands of transformed plants. From these, over 30 dominant mutants with various phenotypes have been isolated. Analysis of a subset of mutants has shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers, at distances ranging from 380 bp to 3.6 kb. In at least one case, the CaMV 35S enhancers led primarily to an enhancement of the endogenous expression pattern rather than to constitutive ectopic expression, suggesting that the CaMV 35S enhancers used here act differently than the complete CaMV 35S promoter. This has important implications for the spectrum of genes that will be discovered by this method.
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openalex_W2141660382 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Detlef Weigel, Ji Hoon Ahn, Miguel Á. Blázquez, Justin Borevitz, S. Christensen, Christian Fankhauser, Cristina Ferrándiz, Igor Kardailsky, Elizabeth J. Malancharuvil, Michael M. Neff, Jasmine Nguyen, Shusei Sato, Zhiyong Wang, Yiji Xia, Richard A. Dixon, Maria Harrison, Chris Lamb, Martin F. Yanofsky, Joanne Chory
Journal Plant physiology and biochemistry : PPB
Year 2000
DOI
10.1104/pp.122.4.1003
URL
https://academic.oup.com/plphys/article-pdf/122/4/1003/38671611/plphys_v122_4_1003.pdf
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