Genome Engineering ofDrosophilawith the CRISPR RNA-Guided Cas9 Nuclease

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ID: 298540
2013
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Abstract
Abstract We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
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Authors Scott J. Gratz, Alexander M Cummings, Jennifer Nguyen, Danielle C. Hamm, Laura K. Donohue, Melissa M. Harrison, Jill Wildonger, Kate M. O’Connor-Giles
Journal current genetics
Year 2013
DOI
10.1534/genetics.113.152710
URL
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