Improving enzymatic hydrolysis of acid-pretreated bamboo residues using amphiphilic surfactant derived from dehydroabietic acid.

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ID: 29678
2019
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Abstract
In this work, amphiphilic surfactant was obtained using dehydroabietic acid from pine rosin and then pre-adsorbed with acid-pretreated bamboo residues (AP-BR) to block the residual lignin adsorption site, which is expected to improve its enzymatic digestibility. Results from cryogenic-transmission electron microscopy (Cryo-TEM) indicated amphiphilic surfactant with PEG with polymerization degree of 34 (D-34) aggregated to form worm-like micelles, which improved enzymatic hydrolysis yield of AP-BR from 24.3% to 71.9% by pre-adsorbing with 0.8 g/L. Amphiphilic surfactants pre-adsorbed on AP-BR could reduce hydrophobicity of AP-BR, adsorption affinity and adsorption capacity of lignin for cellulase from 0.51 L/g to 0.48-0.32 L/g, from 2.9 mL/mg to 1.8-1.4 mL/mg, and from 122.3 mg/g to 101.9-21.4 mg/g, respectively. These changed properties showed compelling positive contributions (R > 0.9) for free enzymes in the supernatants and sequently for final enzymatic hydrolysis yield, which was caused by blocking non-productively hydrophobic adsorption between lignin and cellulase.
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lin2019improvingbioresource Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lin, Wenqian;Chen, Dengfeng;Yong, Qiang;Huang, Caoxing;Huang, Shenlin;
Journal Bioresource technology
Year 2019
DOI
S0960-8524(19)31285-4
URL
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