Use of Polyethylene Glycol to Separate Free and Antibody-Bound Peptide Hormones in Radioimmunoassays†

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ID: 294959
1971
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Abstract
Aqueous polyethylene glycol causes precipitation of antibody-bound peptide hormones labeled with radioactive iodine with little or no precipitation of free hormones. Based on this finding, a method of separation has been developed and applied to radioimmunoassays of insulin, parathyroid hormone, growth hormone and arginine vasopressin. The new method provides several advantages over the double-antibody precipitation method. It appears particularly valuable in immunoassays of substances of low molecular weight.
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openalex_W2164884154 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Bernard Desbuquois, G D Aurbach
Journal the journal of clinical endocrinology & metabolism
Year 1971
DOI
10.1210/jcem-33-5-732
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