Overexpression of [delta]-Pyrroline-5-Carboxylate Synthetase Increases Proline Production and Confers Osmotolerance in Transgenic Plants

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ID: 294839
1995
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Abstract
Proline (Pro) accumulation has been correlated with tolerance to drought and salinity stresses in plants. Therefore, overproduction of Pro in plants may lead to increased tolerance against these abiotic stresses. To test this possibility, we overexpressed in tobacco the mothbean [delta]-pyrroline-5-carboxylate synthetase, a bifunctional enzyme able to catalyze the conversion of glutamate to [delta]-pyrroline-5-carboxylate, which is then reduced to Pro. The transgenic plants produced a high level of the enzyme and synthesized 10- to 18-fold more Pro than control plants. These results suggest that activity of the first enzyme of the pathway is the rate-limiting factor in Pro synthesis. Exogenous supply of nitrogen further enhanced Pro production. The osmotic potentials of leaf sap from transgenic plants were less decreased under water-stress conditions compared to those of control plants. Overproduction of Pro also enhanced root biomass and flower development in transgenic plants under drought-stress conditions. These data demonstrated that Pro acts as an osmoprotectant and that overproduction of Pro results in the increased tolerance to osmotic stress in plants.
Reference Key
openalex_W1832710699 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors PBK. Kishor, Zonglie Hong, Guo-Hua Miao, C.‐A. Hu, D. P. S. Verma
Journal Plant physiology and biochemistry : PPB
Year 1995
DOI
10.1104/pp.108.4.1387
URL
https://doi.org/10.1104/pp.108.4.1387
Keywords Keywords not found

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