A stable genomic source of P element transposase in Drosophila melanogaster.

Clicks: 3
ID: 294580
1988
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Abstract
Abstract A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable. Its transposase activity is higher than that of an entire P strain, but it rarely undergoes internal deletion, excision or transposition. This element was constructed by F. Laski, D. Rio and G. Rubin for other purposes, but we have found it to be useful for experiments involving P elements. We demonstrate that together with a chromosome bearing numerous nonautonomous elements it can be used for P element mutagenesis. It can also substitute efficiently for "helper" plasmids in P element mediated transformation, and can be used to move transformed elements around the genome.
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openalex_W1910936647 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hugh M. Robertson, Christine R Preston, R W Phillis, Dena M. Johnson-Schlitz, Wendy Benz, William R. Engels
Journal current genetics
Year 1988
DOI
10.1093/genetics/118.3.461
URL
https://doi.org/10.1093/genetics/118.3.461
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