Vertical capacitance aptasensors for real-time monitoring of bacterial growth and antibiotic susceptibility in blood.

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ID: 29405
2019
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Ranked #59 of 85 articles by views in Biosensors & bioelectronics

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Abstract
For the treatment of bacteremia, early diagnosis and rapid antibiotic susceptibility tests (ASTs) are necessary because survival chances decrease significantly if the proper antibiotic administration is delayed. However, conventional methods require several days from blood collection to AST as it requires three overnight cultures, including blood culture, subculture, and AST culture. Herein, we report a more rapid method of sensing bacterial growth and AST in blood based on a vertical capacitance sensor functionalized with aptamers. Owing to their vertical structure, the influence of blood cells sunk by gravity on capacitance measurements were minimized. Thus, bacterial growth in blood at 10-10 CFU/mL was monitored in real-time by measuring changes in capacitance at f = 10 kHz. Moreover, real-time capacitance measurements at f = 0.5 kHz provided information on biofilm formation induced during blood cultures. Bacterial growth and biofilm formation are inhibited above the minimal inhibitory concentration of antibiotics; therefore, we also demonstrated that vertical capacitance aptasensors could be applied to rapid AST from positive blood cultures without a need for the subculture process.
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song2019verticalbiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Song, Jun Ho;Lee, Sun-Mi;Park, In Ho;Yong, Dongeun;Lee, Kyo-Seok;Shin, Jeon-Soo;Yoo, Kyung-Hwa;
Journal Biosensors & bioelectronics
Year 2019
DOI
S0956-5663(19)30702-X
URL
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