Rapid preparation of DNA from filamentous fungi

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ID: 291516
1985
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Ranked #44 of 61 articles by views in letters in applied microbiology

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Abstract
We describe a general, simple and inexpensive method for the isolation of DNA from filamentous fungi. Starting from freeze-dried mycelium 01–015% by weight can be isolated as high molecular weight DNA suitable for restriction and ligation in 2 h. The preparation can be done in Eppendorf tubes and allows the processing of many samples in parallel. We have used the method with the basidiomycetes Phanerochaete chrysosporium, Coprinus cinereus and the ascomycete Aspergillus nidulans and others have used it with Trichoderma reesei, Aspergillus niger and for the isolation of DNA from tomato plants.
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openalex_W2156505503 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Ute Raeder, Paul Broda
Journal letters in applied microbiology
Year 1985
DOI
10.1111/j.1472-765x.1985.tb01479.x
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