Genetic applications of an inverse polymerase chain reaction.

Clicks: 1
ID: 291195
1988
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #231 of 320 articles by views in current genetics

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 320 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Abstract A method is presented for the rapid in vitro amplification of DNA sequences that flank a region of known sequence. The method uses the polymerase chain reaction (PCR), but it has the primers oriented in the reverse direction of the usual orientation. The template for the reverse primers is a restriction fragment that has been ligated upon itself to form a circle. This procedure of inverse PCR (IPCR) has many applications in molecular genetics, for example, the amplification and identification of sequences flanking transposable elements. In this paper we show the feasibility of IPCR by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.
Reference Key
openalex_W2159686532 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Howard Ochman, Anne S. Gerber, Daniel L. Hartl
Journal current genetics
Year 1988
DOI
10.1093/genetics/120.3.621
URL
https://doi.org/10.1093/genetics/120.3.621
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.