Coated Charcoal Immunoassay of Insulin

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ID: 290076
1965
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Ranked #183 of 451 articles by views in the journal of clinical endocrinology & metabolism

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Abstract
Charcoal premixed with dextran of average molecular weight 80,000 almost instantly adsorbs free insulin but rejects antibody-bound insulin. The use of such dextran-coated charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, using radioisotope dilution with 131I-insulin and "biopsy" of the insulin pool by antibody to insulin. The procedure here described yields a straight line graph when insulin added is plotted against insulin recovered.
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openalex_W2078073869 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors
Journal the journal of clinical endocrinology & metabolism
Year 1965
DOI
10.1210/jcem-25-10-1375
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