Coated Charcoal Immunoassay of Insulin
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ID: 290076
1965
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Abstract
Charcoal premixed with dextran of average molecular weight 80,000 almost instantly adsorbs free insulin but rejects antibody-bound insulin. The use of such dextran-coated charcoal makes simpler and more rapid the immunoassay of insulin in biologic fluids, using radioisotope dilution with 131I-insulin and "biopsy" of the insulin pool by antibody to insulin. The procedure here described yields a straight line graph when insulin added is plotted against insulin recovered.
| Reference Key |
openalex_W2078073869
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|---|---|
| Authors | Victor Herbert, Kam‐Seng Lau, Chester W. Gottlieb, Sheldon J. Bleicher |
| Journal | the journal of clinical endocrinology & metabolism |
| Year | 1965 |
| DOI |
10.1210/jcem-25-10-1375
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| URL | |
| Keywords | Keywords not found |
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