Local 3D matrix confinement determines division axis through cell shape.

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ID: 28789
2016
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Abstract
How the division axis is determined in mammalian cells embedded in three-dimensional (3D) matrices remains elusive, despite that many types of cells divide in 3D environments. Cells on two-dimensional (2D) substrates typically round up completely to divide. Here, we show that in 3D collagen matrices, mammalian cells such as HT1080 human fibrosarcoma and MDA-MB-231 breast cancer cells exhibit division modes distinct from their Counterparts on 2D substrates, with a markedly higher fraction of cells remaining highly elongated through mitosis in 3D matrices. The long axis of elongated mitotic cells accurately predicts the division axis, independently of matrix density and cell-matrix interactions. This 3D-specific elongated division mode is determined by the local confinement produced by the matrix and the ability of cells to protrude and locally remodel the matrix via β1 integrin. Elongated division is readily recapitulated using collagen-coated microfabricated channels. Cells depleted of β1 integrin still divide in the elongated mode in microchannels, suggesting that 3D confinement is sufficient to induce the elongated cell-division phenotype.
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he2016localoncotarget Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors He, Lijuan;Chen, Weitong;Wu, Pei-Hsun;Jimenez, Angela;Wong, Bin Sheng;San, Angela;Konstantopoulos, Konstantinos;Wirtz, Denis;
Journal oncotarget
Year 2016
DOI
10.18632/oncotarget.5848
URL
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