CRISPR Disruption of Resulted in the Failure of Emergence and Affected the Wing and Gonad Development in the Silkworm .

Clicks: 175
ID: 28731
2019
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Steady

Ranked #31 of 36 articles by views in Insects

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
The domesticated silkworm is an economically important insect that is widely used as a lepidopteran insect model. Although somatic sex determination in the silkworm is well characterized, germline sex determination is not. Here, we used the transgenic-based CRISPR/Cas9 genome editing system to study the function of the gene in . is the homolog of a factor important in germline sex determination in . mutants had abnormally shaped eggs that were disordered in the ovarioles, and gonad development was abnormal. Interestingly, wing discs and wings did not develop properly, and most of the mutants failed to eclose. Gene expression analyses by qRT-PCR showed that gene was highly expressed in the wing disc and epidermis. Genes involved in the WNT signaling pathway and wing development genes and were downregulated in the mutants when compared with wild-type animals. These results demonstrate that the gene product plays an important role in wing metamorphosis. Thus, this study provides new insights into the multiple functions of beyond germline sex determination.
Reference Key
bi2019crisprinsects Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Bi, Honglun;Xu, Xia;Li, Xiaowei;Zhang, Yong;Huang, Yongping;Li, Kai;Xu, Jun;
Journal Insects
Year 2019
DOI
E254
URL
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.