Phenotype and gene-editing evaluation of Cas9-GFP expressing daphnia magna

Clicks: 4
ID: 285057
2024
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #458 of 3,757 articles by views in Malay Journal

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 3,757 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Daphnia magna is a valuable model organism in environmental toxicology, but efficient gene-editing tools are crucial for elucidating gene function. This study evaluated a novel Cas9-GFP expressing D. magna strain for CRISPR-based gene editing. We assessed the transgenic phenotype by comparing growth rates and fecundity with wild-type strain, finding minimal impact on overall fitness. Cas9 expression was confirmed in embryos at 9 hours post-development. To optimize gene editing efficiency, we targeted the scarlet gene (DapmaST), responsible for eye pigmentation, using both capped and 2'-O-methylated gRNAs. While capped gRNA resulted in partial loss of eye pigmentation, methylated gRNA achieved complete loss, demonstrating superior editing efficacy. This study validates the functionality of our Cas9-GFP expressing D. magna model and highlights the importance of gRNA modification in enhancing CRISPR efficiency. Our findings establish an improved system for loss-of-function studies in D. magna, advancing its utility in environmental and genetic research.
Reference Key
persistent_1760654499_68f174a3038b8 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Lumio, Rob T.
Journal Malay Journal
Year 2024
DOI
DOI not found
URL
https://animorepository.dlsu.edu.ph/etdm_chem/21
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.