Electrochemical histamine sensor based on nickel hexacyanoferrate-modified glassy carbon electrode

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ID: 285053
2025
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Abstract
Histamine, which is produced during bacterial degradation of seafoods, is an indicator of food spoilage. Allergic reactions to histamine, especially in seafood products, are potentially life-threatening to some people. In this study, we report on a non-enzymatic histamine sensor based on a nickel hexacyanoferrate-modified glassy carbon electrode (GC/NiHCF). The morphology and composition of the modified electrode were characterized by SEM, EDS, and FTIR. Using the GC/NiHCF electrode, the oxidation potential of histamine starts from 1.1V. The developed electrode used in the electrochemical impedance spectroscopy (EIS) technique was able to increase the response of histamine from 15.56% for the bare electrode to 42.27% for the modified electrode. The modified electrode was also proven to be selective to histamine in the presence of interfering species such as histidine and glycine. The modified electrode exhibited a linear range from 0.75 to 5.0 ppm with a limit of detection determined at 329 ppb. The GC/NiHCF electrode showed a satisfactory recovery performance of 101.13% and 103.32% for yellow fin tuna and mackerel, respectively. The sensor was able to retain 83.1% of its initial response after 150 continuous histamine measurements. Comparison of the EIS technique using the GC/NiHCF electrode with the standard fluorometry technique showed 1.62% and 1.09% difference in detecting histamine in yellowfin tuna and mackerel, respectively. Overall, the GC/NiHCF electrode in EIS was able to demonstrate effectiveness in detecting histamine in real fish samples. Keywords: histamine, electrochemical impedance spectroscopy, histamine sensor
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persistent_1760654487_68f17497c4b70 Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mansilungan, Miguel D
Journal Malay Journal
Year 2025
DOI
DOI not found
URL
https://animorepository.dlsu.edu.ph/etdm_chem/30
Keywords Keywords not found

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