Simultaneous Measurement of Viscosity and Optical Density of Bacterial Growth and Death in a Microdroplet.

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ID: 28503
2018
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Abstract
Herein, we describe a novel method for the assessment of droplet viscosity moving inside microfluidic channels. The method allows for the monitoring of the rate of the continuous growth of bacterial culture. It is based on the analysis of the hydrodynamic resistance of a droplet that is present in a microfluidic channel, which affects its motion. As a result, we were able to observe and quantify the change in the viscosity of the dispersed phase that is caused by the increasing population of interacting bacteria inside a size-limited system. The technique allows for finding the correlation between the viscosity of the medium with a bacterial culture and its optical density. These features, together with the high precision of the measurement, make our viscometer a promising tool for various experiments in the field of analytical chemistry and microbiology, where the rigorous control of the conditions of the reaction and the monitoring of the size of bacterial culture are vital.
Reference Key
sklodowska2018simultaneousmicromachines Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Sklodowska, Karolina;Debski, Pawel R;Michalski, Jacek A;Korczyk, Piotr M;Dolata, Miroslaw;Zajac, Miroslaw;Jakiela, Slawomir;
Journal micromachines
Year 2018
DOI
E251
URL
Keywords
reproductive performance hydrogen sulfide methionine placental angiogenesis pregnant sows

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