Preparation and evaluation of bean-derived liprotide nanocarriers to enhance curcumin cytotoxicity against HT-29 colorectal cancer cells

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ID: 284989
2025
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Abstract
Cancer is a dynamic and complex disease that has caused the death of millions and begs ever more intensive research. This study contributed to these efforts by synthesizing a novel type of nanocomplex, liprotides, from mung and soybean proteins with the objective of delivering curcumin to colorectal cancer cells. The proteins were extracted through alkaline and isoelectric precipitation methods, then separated into their albumin (mung albumin, MA, and soy albumin, SA) and globulin (mung globulin, MG, and soy globulin, SG) fractions, following quantification by the Bradford assay. These protein fractions were then mixed with oleic acid under mild heating conditions (80℃) to synthesize the target core-shell complexes: mung albumin liprotides (MAL), mung globulin liprotides (MGL), soy albumin liprotides (SAL), and soy globulin liprotides (SGL). Successful complexation was denoted by changes in the IR spectra and secondary structure distributions, increased denaturation temperatures, and apparent turbidity changes in each liprotide solution compared to its protein starting material. The complexes were then combined with curcumin to achieve encapsulation of the drug by spontaneous hydrophobic interactions, where MAL showed the highest encapsulation at 91% efficiency, followed by SAL at 65.09%, SGL at 60.60%, and MGL at 59.58%. Transmission electron micrographs of the curcumin-loaded MAL and SAL showed evidence of encapsulation by increased particle diameter and hyperpigmentation of the core structure. Finally, the cytotoxicity of each liprotide and its curcumin inclusion complexes against HT-29 colorectal adenocarcinoma cell line was investigated. The SGL treatment showed significant evidence of cytotoxic activity, resulting in cell viability reduction to 67.43 ± 1.01% alone, while upon the inclusion of curcumin, cell viability was further depleted to 61.33 ± 2.68% after 24 hours.
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Authors Enriquez, Isabel Beatrice A.
Journal Malay Journal
Year 2025
DOI
DOI not found
URL
https://animorepository.dlsu.edu.ph/etdb_chem/59
Keywords Keywords not found

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