An analysis on the variability of the tilapia lake virus (TiLV) whole genome to aid in detection and treatment target
Clicks: 4
ID: 284765
2023
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
0.9
/100
4 views
0 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #252 of 3,757 articles by views in Malay Journal
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 3,757 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Tilapia is one of the most important farmed fish worldwide for its affordability, marketability, adaptability, and hardiness in resisting disease. However, huge losses of cultured tilapia in an Israeli tilapia farm led to the discovery of the tilapia lake virus (TiLV) in 2014. It has since spread to four (4) continents and sixteen (16) countries, causing a huge threat to the global tilapia aquaculture industry for its high mortality rates reaching up to 90%. As a novel virus, much of its basic foundation is still unknown, including its genetic information and variability that has led to its rapid spread. Thus, the study aims to learn about the genetic variability of the TiLV genome to aid in detection and treatment target. An evaluation on the genomic variability was conducted through MEGA11 on twenty-two (22) TiLV genomes collected from eight (8) countries over a decade. It was found through pairwise distance estimation that segments 9 and 10 of the TiLV genome have remained largely conserved, making it a good target for virus detection. Four (4) primers each for both segments were chosen out of seventy-one (71) designed primers through NCBI Primer-BLAST with a GC% content between 50-60 and specificity for selected strains of well-supported nodes in the segments’ phylogenetic tree (lnL = -1116.19 and -1162.96, bootstrap valuesremoved). For the treatment target, the most conserved regions could not be fully evaluated due to the unknown properties of the hypothetical proteins, so the relatively conserved segment 1 coding for the putative PB1 gene was determined as the best treatment target. It was also found that the nucleotide frequency of a strain from Israel collected in 2011 (Til-4-2011) displayed a vastly different pattern compared to the rest of the sequences. The findings of this study are significant as it was able to establish certain genetic information that was previously lacking, including the genetic variation between the segments to determine the most conserved and varied regions, as well as the nucleotide compositions between the different strains. Geographical origin was also found to play a role in the reassortment of the virus as well-supported clades tended to come from the same region.
| Reference Key |
persistent_1760653617_68f17131ab314
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | Lontok, Rain Allisha M. |
| Journal | Malay Journal |
| Year | 2023 |
| DOI |
DOI not found
|
| URL | |
| Keywords | Keywords not found |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.