Cytotoxicity of auricularia polytricha and pleurotus ostreatus var. florida ethanol extracts on human cancer cell lines

Clicks: 1
ID: 284716
2024
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal

Ranked #3,526 of 3,757 articles by views in Malay Journal

Most read Least read

Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 3,757 in total.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Cancer arises from a multitude of genetic alterations, leading to a spectrum of clinical manifestations that pose a challenge to the global healthcare system. In recent years, there has been a growing trend in the exploration of alternative approaches that leverage the potential of mushrooms as anticancer treatments. Therefore, this study sought to evaluate the cytotoxic potential of crude ethanolic extracts derived from the mushrooms Auricularia polytricha and Pleurotus ostreatus var. florida against human and cancer cell lines using the PrestoBlue™ cytotoxicity assay. The extraction of polar and non-polar compounds from A. polytricha and P. ostreatus var. florida was performed using ethanol. The crude ethanolic extracts were subsequently tested for their cytotoxicity on pancreatic adenocarcinoma (BxPC-3), lung small cell carcinoma (H69PR), and normal liver epithelial (THLE-3) cell lines, which were exposed for 72 hours to two-fold serial dilutions of the extracts having a concentration range of 15.625 to 2000 µg/mL. Zeocin™ served as the positive control. The absorbances of the culture plates were measured at 570 nm and 600 nm, and their cytotoxicity indices were determined to calculate the half-maximal inhibitory concentration (IC50) of the extracts on the human cell lines. Zeocin™ exhibited cytotoxic activity against all the cell lines, with IC50 values of 585.4 μg/mL, 151.4 μg/mL, and 377.5 μg/mL for BxPC-3, H69PR, and THLE-3, respectively. Contrastingly, both mushroom extracts had IC50 values greater than 2,000 µg/mL, which was the highest concentration tested. While the current findings may not indicate robust cytotoxic effects, this does not preclude the potential for these treatments to possess cytotoxic activity.
Reference Key
persistent_1760653476_68f170a4018cd Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Obrero, Ariel Iverson Betita
Journal Malay Journal
Year 2024
DOI
DOI not found
URL
https://animorepository.dlsu.edu.ph/etdb_bio/84
Keywords Keywords not found

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.