Sonication-assisted osmotic shock for the decellularization of porcine skin for tissue engineering applications

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ID: 284699
2025
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Abstract
Decellularization hinges on two fundamental, often conflicting, priorities: complete cell removal and the preservation of extracellular matrix (ECM) structure and components. While ionic and nonionic detergents are frequently employed for efficient cell removal, they can compromise ECM structure and leave cytotoxic residues. This study presents a detergent-free, sonication-assisted approach to decellularization, aiming to balance effective cell removal with the preservation of ECM integrity. Epidermis were subjected to alternating hypertonic and hypotonic solutions, along with sonication at 60 W, 120 W, and 180 W in a bath-type sonicator. Results revealed that lower sonication power (60 W) was more effective in decellularizing porcine skin, demonstrating 84.54% reduction in cellular debris, compared to 25.76% and 70.71% reductions for 120 W and 180 W, respectively, as it maintained a favorable balance between cellular disruption and ECM preservation. Hematoxylin and eosin staining results indicated that excessive sonication intensity led to significant ECM damage, resulting in a denser matrix that retained cellular debris. Moreover, Attenuated Total Reflectance Fourier-Transform Infrared (ATR-FTIR) Spectroscopy revealed characteristic amide-associated bands indicating protein and collagen presence, with treated samples exhibiting increased transmittance, suggesting alterations in functional group concentration and protein structure. Although increasing sonication power enhanced cellular disruption, it also induced excessive cavitation, which compromised ECM integrity. This study demonstrates the potential of a novel, detergent-free decellularization method that effectively removes cells while preserving the structural integrity of the extracellular matrix, offering significant advancements in tissue engineering and regenerative medicine.
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Authors Portento, Hanna Nicole P.
Journal Malay Journal
Year 2025
DOI
DOI not found
URL
https://animorepository.dlsu.edu.ph/etdb_bio/99
Keywords Keywords not found

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