GC-MS Profile of Diodella sarmentosa (SW) Bacigalupo El Cabral ex Borhidi Ethanol Leaf Extract and its Total Dehydrogenase Inhibitory Potential

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ID: 281711
2021
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Abstract
Phytochemical composition of ethanol leaf extract of Diodella sarmentosa was profiled with GC-MS and the inhibitory property of the extract against total microbial dehydrogenases were assessed. The major constituents of the extract were squalene (29.50%), Phytol (24.68%), phenol, 3-pentadecyl- (18.58%), 1-Butanol, 3-methyl- (9.09%) and n-Hexadecanoic acid (7.78%). The minimum inhibitory concentration (MIC) of the extract against broad spectrum of microbial population was assessed. Bacillus subtilis, Candidas spp, and Penicillium spp were more sensitive to the treatment and thus; were further investigated using Dehydrogenase activity assay method. Total dehydrogenase activities of Bacillus subtilis, Candidas spp, and Penicillium spp at the extract concentration range of 0 to 2000mg/ml were progressively inhibited at increasing extract concentrations. The threshold inhibitory concentrations (IC50) of the extracts against Candidas spp, Penicillium spp and Bacillus subtilis were 275micro. g/ml, 322 micro. g/ml and 411 micro. g/ml respectively. Our findings suggested the extract as a useful source of antimicrobial phytochemicals for pharmaceutical use.
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ezerioha2021gcms Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Callistus Izunna Iheme; Doris Ifeyinwa Ukairo; Okechukwu Igwe Oguoma; Linus Ahumareze Nwaogu; Amanda Ugochi Ezirim; Chiamaka Perpetua Nzebude; Chidimma Caryn Ezerioha
Journal arXiv
Year 2021
DOI
DOI not found
URL
http://arxiv.org/abs/2102.04194v1
Keywords
q-bio.qm

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