Amplicon-based next-generation sequencing reveals the co-existence of multiple Leishmania species in patients with visceral leishmaniasis.

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ID: 280759
2022
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Ranked #66 of 68 articles by views in International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

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Abstract
Visceral leishmaniasis (VL) is a mammalian protozoal disease propagated in the Americas by female phlebotomine sandflies, mainly caused by Leishmania infantum. However, in recent years, cases of VL caused by different Leishmania species, such as L. amazonensis and L. colombiensis, have been reported in the continent. This study used an amplicon-based next-generation sequencing approach to identify VL aetiologic species using high-depth sequencing targeting a region on the Heat Shock Protein 70 gene. In this first approach, six samples from five patients diagnosed with VL were selected and analysed to identify DNA of Leishmania spp. All samples harboured DNA of L. infantum; five samples were found to be co-infected with other Leishmania spp. or with Trypanosoma cruzi, and just one sample was mono-infected with L. infantum. This study demonstrates the usefulness of this methodology to identify trypanosomatid co-infections in clinical samples, which presents an interesting study panorama considering their biological, clinical and epidemiological implications.
Reference Key
castillocastaeda2022ampliconbasedinternational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Castillo-Castañeda, Adriana;Patiño, Luz H;Muñoz, Marina;Ayala, Martha S;Segura, Maryi;Bautista, Jessica;Shaban, Maryia V;Paniz-Mondolfi, Alberto;Ramírez, Juan David;
Journal International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases
Year 2022
DOI
10.1016/j.ijid.2021.11.029
URL
Keywords
leishmania hsp70 visceral leishmaniasis co-infection amplicon-based ngs trypanosomatid

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