A portable CRISPR-Cas12a triggered photothermal biosensor for sensitive and visual detection of Staphylococcus aureus and Listeria monocytogenes.

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ID: 278735
2024
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Abstract
The detection of foodborne pathogens is crucial for ensuring the maintenance of food safety. In the present study, a portable CRISPR-Cas12a triggered photothermal biosensor integrating branch hybrid chain reaction (bHCR) and DNA metallization strategy for sensitive and visual detection of foodborne pathogens was proposed. The sheared probes were utilized to block the locker probes, which enabled preventing the assembly of bHCR in the absence of target bacteria, while target bacteria can activate the cleavage of sheared probes through CRISPR-Cas12a. Therefore, the locker probes functioned as initiating chains, triggering the formation of the branching double-stranded DNA consisting of H1, H2, and H3. The silver particles, which were in situ deposited on the DNA structure, functioned as a signal factor for conducting photothermal detection. Staphylococcus aureus and Listeria monocytogenes were selected as the foodborne pathogens to verify the analytical performance of this CRISPR-Cas12a triggered photothermal sensor platform. The sensor exhibited a sensitive detection with a low detection limit of 1 CFU/mL, while the concentration ranged from 10 to 10 CFU/mL. Furthermore, this method could efficiently detect target bacteria in multiple food samples. The findings demonstrate that this strategy can serve as a valuable reference for the development of a portable platform enabling quantitative analysis, visualization, and highly sensitive detection of foodborne bacteria.
Reference Key
gu2024atalanta Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Gu, Xijuan;Tang, Qu;Kang, Xiaoxia;Ji, Huoyan;Shi, Xiuying;Shi, Linyi;Pan, Anli;Zhu, Yidan;Jiang, Wenjun;Zhang, Jing;Liu, Jinxia;Wu, Mingmin;Wu, Li;Qin, Yuling;
Journal Talanta
Year 2024
DOI
10.1016/j.talanta.2024.125678
URL
Keywords
food safety crispr-cas12a dna metallization photothermal biosensor

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