Generation and characterization of nanobodies targeting human pepsinogens.

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ID: 278015
2024
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Abstract
Human pepsinogens (mainly pepsinogen I and pepsinogen II) are the major inactive precursor forms of the digestive enzyme pepsin which play a crucial role in protein digestion. The levels and ratios of human pepsinogens have demonstrated potential as diagnostic biomarkers for gastrointestinal diseases, particularly gastric cancer. Nanobodies are promising tools for the treatment and diagnosis of diseases, owing to their unique recognition properties. In this study, recombinant human pepsinogens proteins were expressed and purified as immunized antigens. We constructed a VHH phage library and identified several nanobodies via phage display bio-panning. We determined the binding potency and cross-reactivity of these nanobodies. Our study provides technical support for developing immunodiagnostic reagents targeting human pepsinogens.
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zhang2024generationprotein Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhang, Shenglan;Ma, Jieyao;He, Liu;Li, Qianying;He, Pan;Li, Jing;Zhang, Huicong;
Journal Protein expression and purification
Year 2024
DOI
10.1016/j.pep.2024.106431
URL
Keywords
nanobody single domain antibody pgi pgii pepsinogens

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