The expression of HIV-1 tat in Lactococcus lactis.

Clicks: 117
ID: 278001
2024
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Abstract
Efficient expression of functional proteins in heterologous hosts has become the pivotal focus of modern biotechnology and biomedical research. To this end, multiple alternatives to E. coli are being explored for recombinant protein expression. L. lactis, being a gram-positive organism, circumvents the need for an endotoxin removal step during protein purification. We report here the optimisation of the expression of HIV-1 Tat, a notoriously difficult protein, in Lactococcus lactis system. We evaluated five different promoters in two different Lactococcus lactis strains and examined the effect of pH, glucose, and induction time on the yield and purity of Tat. Finally, the recombinant Tat was functionally competent in transactivating the HIV-1 promoter in HLM-1 reporter cells. Our work provides a scaffold for future work on the expression of toxic proteins in Lactococcus lactis.
Reference Key
selvam2024theprotein Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Selvam, Deepak;D'silva, Anish;Panchapakesan, Arun;Gohil, Yuvrajsinh;Singh, Jayendra;Hanna, Luke Elizabeth;Ranga, Udaykumar;
Journal Protein expression and purification
Year 2024
DOI
10.1016/j.pep.2024.106443
URL
Keywords
endotoxin hiv-1 tat protein expression lactococcus lactis p2 promoter

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