Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells.

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2019
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Abstract
Multimodal single-cell assays provide high-resolution snapshots of complex cell populations, but are mostly limited to transcriptome plus an additional modality. Here, we describe expanded CRISPR-compatible cellular indexing of transcriptomes and epitopes by sequencing (ECCITE-seq) for the high-throughput characterization of at least five modalities of information from each single cell. We demonstrate application of ECCITE-seq to multimodal CRISPR screens with robust direct single-guide RNA capture and to clonotype-aware multimodal phenotyping of cancer samples.
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mimitou2019multiplexednature Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mimitou, Eleni P;Cheng, Anthony;Montalbano, Antonino;Hao, Stephanie;Stoeckius, Marlon;Legut, Mateusz;Roush, Timothy;Herrera, Alberto;Papalexi, Efthymia;Ouyang, Zhengqing;Satija, Rahul;Sanjana, Neville E;Koralov, Sergei B;Smibert, Peter;
Journal Nature Methods
Year 2019
DOI
10.1038/s41592-019-0392-0
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