Comparative Structural and Antigenic Characterization of Genetically Distinct -Polysaccharides.

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ID: 2742
2019
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Abstract
Little is known about the underlying basis of serotype specificity among strains of , the agent of rainbow trout fry syndrome and bacterial cold-water disease. The identification of different heat-stable O-serotypes among strains of this gram-negative pathogen does, however, suggest structural variations in the -polysaccharide (O-PS) moiety of cell surface lipopolysaccharide (LPS). A trisaccharide composed of L-rhamnose (L-Rha), 2-acetamido-2-deoxy-L-fucose (L-FucNAc) and 2-acetamido-4-R-2,4-dideoxy-D-quinovose (D-Qui2NAc4NR), where R represents a dihydroxyhexanamido derivative, was previously identified as the repeating unit of CSF259-93 O-PS. Interestingly, the O-PS gene cluster of this strain and that of 950106-1/1, which belongs to a different O-serotype, are identical except for , which encodes the putative polymerase that links trisaccharide repeats into O-PS chains. We have now found from results of glycosyl composition analysis and high-resolution nuclear magnetic resonance, that the linkage of D-Qui2NAc4NR to L-Rha, which is α1-2 for CSF259-93 versus β1-3 for 950106-1/1, is the only structural difference between O-PS from these strains. The corresponding difference in O-serotype specificity was established from the reactions of rabbit and trout anti- antibody with purified O-PS and LPS. Moreover, LPS-based differences in antigenicity were noted between strains with O-PS loci identical to those of CSF259-93 or 950106-1/1, except for the genes predicted to direct synthesis of different R-groups in Qui2NAc4NR. The findings provide a framework for defining the genetic basis of O-PS structure and antigenicity and suggest that the repertoire of O-serotypes extends beyond what is presently recognized from serological studies of this important fish pathogen.
Reference Key
cisar2019comparativefrontiers Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Cisar, John O;Bush, C Allen;Wiens, Gregory D;
Journal Frontiers in microbiology
Year 2019
DOI
10.3389/fmicb.2019.01041
URL
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