Vis-Fusion LIEXA: A Point-of-Care Testing Method for Highly Specific and Sensitive Quantitation of Fusion Gene with SmartPhone.

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ID: 27377
2019
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Abstract
Fusion genes, playing a causal role in human tumorigenesis and developments, are deemed as gold standard molecular bi-omarkers in cancer diagnosis, therapy, and prognosis. A rapid, robust and sensitive method detection of fusion gene for point-of-care (POC) diagnosis is urgently needed. Here, taking the advantages of the superior specificity of ligation reaction and highly amplified efficiency of isothermal exponential amplification with pH indicator, we developed a colorimetric method for visual detection of fusion gene with high sensitivity and specificity by the naked eye. More importantly, we first found that fusion gene can be accurately quantified in a wide dynamic range (2 zmol to 2 fmol) by an open-source App with SmartPhone-assisted RGB (Red, Green, and Blue value) reading mode. The proposed method for Visual detection of Fu-sion gene by Ligation-triggered Isothermal Exponential Amplification is termed Vis-Fusion LIEXA. We have demonstrated that the Vis-Fusion LIEXA is a practical and reliable method for accurate quantitative detection of the fusion gene in a complex biological sample at zmol level in 40 min only with a SmartPhone, thereby providing a user-friendly and point-of-care testing (POCT) tool for molecular diagnostics.
Reference Key
wang2019visfusionanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Wang, Hui;Wang, Honghong;Jia, Yuting;Sun, Ruyan;Hong, Weixiang;Zhang, Mai;Li, Zhengping;
Journal Analytical chemistry
Year 2019
DOI
10.1021/acs.analchem.9b03061
URL
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