Vir typing: a long-PCR typing method for group A streptococci

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ID: 272766
1995
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Abstract
We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure …
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Authors Gardiner D;Hartas J;Currie B;Mathews JD;Kemp DJ;Sriprakash KS;;
Journal PCR methods and applications
Year 1995
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/7580916
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE genetic humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't DNA sensitivity and specificity Polymerase Chain Reaction / methods* Base Sequence Molecular Sequence Data Polymorphism bacterial proteins / genetics* streptococcus pyogenes / isolation & purification bacterial / genetics dna primers streptococcal infections / microbiology* streptococcus pyogenes / classification* bacterial / isolation & purification streptococcus pyogenes / genetics streptococcal infections / transmission restriction mapping k s sriprakash virulence factors* pmid:7580916 doi:10.1101/gr.4.5.288 d gardiner j hartas endodeoxyribonucleases / genetics regulon

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